Motility Test Agar - 7247

Item No.  7247A

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Motility Test Agar is used for the differentiation of microorganisms on the basis of motility in a laboratory setting. Motility Test Agar is not intended for use in the diagnosis of disease or other conditions in humans. In 1936, Tittsler and Sandholzer reported using a semisolid agar for the detection of bacterial motility. Motility Test Agar is a modification of this formulation. Bacterial motility is observed macroscopically by a diffuse zone of growth spreading from the line of inoculation. Certain species of motile bacteria will show diffuse growth throughout the entire medium, while others may show diffusion from one or two points appearing as nodular outgrowths along the stab. Tittsler and Sandholzer reported tubes incubated for one day gave identical results with the hanging drop method, and incubation for two days permitted demonstration of motility in an additional 4% of cultures tested. Motility Test Agar is recommended for detection of microbial motility in food and dairy standard methods.  
Brand NEOGEN Culture Media
Formula Liter
Enzymatic Digest of Gelatin 10 g
Beef Extract 3 g
Sodium Chloride 5 g
Agar 4 g
Mass 500 g
Package Weight 0.50 LB
  1. Suspend 22 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.




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