Reveal® 2.0 for E. coli O157:H7 Medium
500 g
SKU No. 700002803 | Catalog No. 9728
| Specifications | ||
|---|---|---|
| Brand | Reveal® | |
| Analyte | E. coli O157:H7 | |
| Platform | Lateral Flow | |
| Result Type | Presence/Absence | |
| Approvals | AOAC-RI 011103 | |
| Limit of Detection | 1 cfu/analytical unit | |
| Testing Time | 10.00 | |
| Time to Result | 8 20 | |
| Mass | 500 g | |
| Quantity per Package | 1 | |
| Package Dimensions | 3.60 in x 3.60 in x 6.50 in | |
| Package Weight | 0.50 lb | |
Documents
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Technology
The system utilizes specified enrichment media to provide E. coli O157:H7 with readily available nutrients and other factors required for its survival and rapid growth. Used with Reveal media, the system can provide results in 12 to 20 hours.
A portion (200 µL) of the enriched culture is placed into the sample cup followed by a chemical treatment. The test device is placed into the sample cup during the chemical treatment and is incubated at 42°C for 15 minutes. The sample is wicked through the reagent zone that contains specific anti-E. coli O157:H7 antibodies conjugated to colloidal gold particles.
If antigens are present in the sample, they will bind to the gold-conjugated antibodies. This antigenantibody complex then leaves the reagent zone and travels across the membrane, which contains a zone of anti-E. coli O157:H7 antibodies. The immune complex with gold conjugate is captured and aggregates in this zone, thus displaying a visible line. The remainder of the sample continues to migrate to the end of the membrane where it is deposited into a waste reservoir.
The reagent zone also contains a gold conjugate of a second antigen, which is eluted by the sample regardless of the presence of E. coli O157:H7 antigen to form a line in the control zone, ensuring the test is working properly.
Training
Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.