Enhanced K-Blue® TMB Substrate

Item No.  308175

  • High activity
  • Longest shelf-life of Neogen TMB substrates
  • Low background
  • Minimum shelf life of 48 months at 2–8°C
This item has shipping restrictions.
$82.90 each
$138.30 each
$253.00 each
$3,288.40 each
In stock
Out of stock
Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Enhanced K-Blue® is a one bottle stabilized chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Enhanced K-Blue develops a deep blue color in the presence of peroxidase labelled conjugate. Enhanced K-Blue has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate.

Request Substrate Sample

Brand K-Blue®
Application Microwell
Intended Use Research and manufacturing use only
Result Type Colorimetric
Enzyme HRP
Sensitivity High Activity
Storage Conditions Stable for a minimum of 48 months when stored at 2-8°C.
Wavelength 650 nm or 450 nm with acid stop
Package Weight 0.50


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training.