K-Blue® Aqueous TMB Substrate

Item No.  331175

  • 100% solvent-free
  • Ideal when working under strict regulatory requirements
  • Compliant with Annex XVII for REACH
  • Low background
  • Minimum shelf life of 36 months at 2–8°C
This item has shipping restrictions.
$78.20 each
$130.50 each
$238.70 each
$3,102.30 each
In stock
Out of stock
Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

K-Blue® Aqueous is a revolutionary 100% solvent-free, non-volatile TMB substrate. It contains 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in a one-bottle format with long-term stability and high activity. K-Blue Aqueous develops a deep blue color in the presence of peroxidase labelled conjugate and is ideal for end users working under strict regulatory requirements. The formula is compliant with Annex XVII for REACH and contains no hazardous substances at or above published reporting levels. K-Blue Aqueous has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate.

Request Substrate Sample

Brand K-Blue®
Application Microwell
Intended Use Research and manufacturing use only
Result Type Colorimetric
Enzyme HRP
Sensitivity Mid-level activity
Storage Conditions Stable for a minimum of 36-months when stored at 2-8°C.
Wavelength 650 nm or 450 nm with acid stop
Package Weight 0.50 lb


To access the Certificate of Analysis (COA), please use our COA Search.

K-Blue Aqueous Substrate - English - US 331171_331175_331176_331177_331199_331257_SDS_da-DK.pdf 331171_331175_331176_331177_331199_331257_SDS_en-AU.pdf 331171_331175_331176_331177_331199_331257_SDS_en-CA.pdf 331171_331175_331176_331177_331199_331257_SDS_en-NZ.pdf 331171_331175_331176_331177_331199_331257_SDS_en-PH.pdf 331171_331175_331176_331177_331199_331257_SDS_es-005.pdf 331171_331175_331176_331177_331199_331257_SDS_es-419.pdf 331171_331175_331176_331177_331199_331257_SDS_es-US.pdf 331171_331175_331176_331177_331199_331257_SDS_fi-FI.pdf 331171_331175_331176_331177_331199_331257_SDS_fr-CA.pdf 331171_331175_331176_331177_331199_331257_SDS_hu-HU.pdf 331171_331175_331176_331177_331199_331257_SDS_id-ID.pdf 331171_331175_331176_331177_331199_331257_SDS_it-IT.pdf 331171_331175_331176_331177_331199_331257_SDS_ja-JA.pdf 331171_331175_331176_331177_331199_331257_SDS_ko.pdf 331171_331175_331176_331177_331199_331257_SDS_ms-MY.pdf 331171_331175_331176_331177_331199_331257_SDS_nl-NL.pdf 331171_331175_331176_331177_331199_331257_SDS_no-NO.pdf 331171_331175_331176_331177_331199_331257_SDS_pl-PL.pdf 331171_331175_331176_331177_331199_331257_SDS_pt-BR.pdf 331171_331175_331176_331177_331199_331257_SDS_sv-SE.pdf 331171_331175_331176_331177_331199_331257_SDS_th-TH.pdf 331171_331175_331176_331177_331199_331257_SDS_vi-VN.pdf 331171_331175_331176_331177_331199_331257_SDS_zh-Hans-CN.pdf 331171_331175_331176_331177_331199_331257_SDS_zh-Hant-TW.pdf K-Blue Aqueous Substrate - Deutsch K-Blue Aqueous Substrate - English - Great Britain K-Blue Aqueous Substrate - Français


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training.