K-Blue® Advanced Plus TMB Substrate

Item No.  379175

  • High activity
  • No hazardous labeling
  • Compliant with Annex XVII for REACH
  • Minimum shelf life of 24 months at 2–8°C
This item has shipping restrictions.
$91.20 each
$152.10 each
$278.40 each
$3,617.40 each
In stock
Out of stock
Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

K-Blue Advanced Plus is a one-bottle stabilized chromogenic substrate for use with horseradish peroxidase immunoassays. It contains both 3,3’,5,5’ tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). K-Blue Advanced Plus develops a deep blue color in the presence of peroxidase labeled conjugate. This substrate has been optimized for use with microwell assays, especially ELISA. K-Blue Advanced Plus is not applicable for use with assays requiring a precipitating substrate. The substrate offers long-term stability and high activity. K-Blue Advanced Plus is compliant with Annex XVII for REACH and contains no hazardous substances at or above published reporting levels.

Request Substrate Sample

Brand K-Blue®
Application Microwell
Intended Use Research and manufacturing use only
Result Type Colorimetric
Enzyme HRP
Sensitivity High Activity
Storage Conditions Stable for a minimum of 24-months when stored at 2-8°C.
Wavelength 650 nm or 450 nm with acid stop
Package Weight 0.50


To access the Certificate of Analysis (COA), please use our COA Search.


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training.