Protease (Subtilisin A from Bacillus licheniformis)

Item No.  E-BSPRT-100ML

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High purity Protease (Subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For use in Megazyme Total Dietary Fiber test method. 

E-BSPRT-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.

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Specifications
Brand Megazyme
EC Number 3.4.21.62
Enzyme Protease
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Application Examples This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fiber analytical procedures.
Formulation In 50% (v/v) glycerol
Physical Form Solution
CAS Number 9014-01-1 
Synonyms subtilisin; subtilisin A
Source Bacillus licheniformis
Molecular Weight 30250
Expression Purified from Bacillus licheniformis
Specificity Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides.
Specific Activity ~ 6 U/mg of protein (40°C, pH 8.0 on casein)
Unit Definition One Unit will hydrolyse casein to produce colour equivalent to one µmole (181 µg) of tyrosine per minute at pH 7.0 at 40°C (colour by Folin-Ciocalteu reagent).
Temperature Optima (°C) 60
pH Optima 7.0-7.4
Package Weight 0.30 lb

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