Protease (Subtilisin A from Bacillus licheniformis)
Item No. 700004201 | Catalog No. E-BSPRT-100ML
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High purity Protease (Subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Total Dietary Fiber test method.
E-BSPRT-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 3.4.21.62 | |
| Enzyme | Protease | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: > 4 years at 4°C |
|
| Application Examples | This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fiber analytical procedures. | |
| Formulation | In 50% (v/v) glycerol | |
| Physical Form | Solution | |
| CAS Number | 9014-01-1 | |
| Synonyms | subtilisin; subtilisin A | |
| Source | Bacillus licheniformis | |
| Molecular Weight | 30250 | |
| Expression | Purified from Bacillus licheniformis | |
| Specificity | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. | |
| Specific Activity | ~ 6 U/mg of protein (40°C, pH 8.0 on casein) | |
| Unit Definition | One Unit will hydrolyse casein to produce colour equivalent to one µmole (181 µg) of tyrosine per minute at pH 7.0 at 40°C (colour by Folin-Ciocalteu reagent). | |
| Temperature Optima (°C) | 60 | |
| pH Optima | 7 | |
| Package Dimensions | 1.70 in x 1.70 in x 3.90 in | |
| Package Weight | 0.30 lb | |
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