Fructan Assay Kit
100 assays per kit
SKU No. 700004285 | Catalog No. K-FRUC
- Very cost effective
- All kit reagents stable for > 2 years after preparation
- Unaffected by high sucrose / reducing sugar concentrations
- Fructan kits are only available from Megazyme
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).
New, improved procedure.
In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.
The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| Analyte | Fructan | |
| Platform | Spectrophotometer | |
| Result Type | UV Absorbance | |
| Approvals | AACC Method 32-32.01, AOAC Method 999.03, AOAC Method 2016.14, AOAC Method 2018.07 and CODEX Method Type III | |
| Limit of Detection | 0.16 g/100 g | |
| Storage Conditions | Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions | |
| Wavelength | 410 nm | |
| Application Examples | Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials | |
| Signal Response | Increase | |
| Linear Range | 2.3 to 55 μg of D-fructose or D-glucose per assay | |
| Total Assay Time | Approx. 90 min | |
| Package Dimensions | 10.00 in x 5.00 in x 2.00 in | |
| Package Weight | 0.46 lb | |
Documents
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