Available Carbohydrates Assay Kit

100 Assays of each per kit

Item No.  700004267  |  Catalog No.  K-AVCHO

This item has shipping restrictions.
$454.00 each
In stock
Out of stock
Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving 'First Action' status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).

* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.

View Documents

Brand Megazyme
Analyte Available Carbohydrates, Dietary Fiber
Platform Spectrophotometer
Result Type UV Absorbance
Approvals AOAC Method 2020.07
Limit of Detection 1.475 g/100 g
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 6 months under recommended storage conditions

Wavelength 340 nm
Application Examples Food ingredients, food products and other materials
Signal Response Increase
Linear Range 4 to 80 μg of D-glucose, D-fructose or D-galactose per assay
Reaction Time ~ 5 h
Package Dimensions 10.00 in x 5.00 in x 2.00 in
Package Weight 0.69 lb


To access certificates, please use our technical document search page.