Available Carbohydrates Assay Kit
100 Assays of each per kit
Item No. K-AVCHO
The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving 'First Action' status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).
* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| Analyte | Available Carbohydrates, Dietary Fiber | |
| Platform | Spectrophotometer | |
| Result Type | UV Absorbance | |
| Approvals | AOAC Method 2020.07 | |
| Limit of Detection | 1.475 g/100 g | |
| Storage Conditions |
Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels Stability: > 2 years under recommended storage conditions |
|
| Wavelength | 340 nm | |
| Application Examples | Food ingredients, food products and other materials | |
| Signal Response | Increase | |
| Linear Range | 4 to 80 μg of D-glucose, D-fructose or D-galactose per assay | |
| Reaction Time | ~ 5 h | |
| Package Weight | 0.69 lb | |
Documents
To access the Certificate of Analysis (COA), please use our COA Search.