Available Carbohydrates Assay Kit

100 Assays of each per kit

Item No.  K-AVCHO

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$358.00 each
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The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving 'First Action' status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).

* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.

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Specifications
Brand Megazyme
Analyte Available Carbohydrates, Dietary Fiber
Platform Spectrophotometer
Result Type UV Absorbance
Approvals AOAC Method 2020.07
Limit of Detection 1.475 g/100 g
Storage Conditions

Storage Temperature: Short term stability: 2-8°C, Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Wavelength 340 nm
Application Examples Food ingredients, food products and other materials
Signal Response Increase
Linear Range 4 to 80 μg of D-glucose, D-fructose or D-galactose per assay
Reaction Time ~ 5 h
Package Weight 0.69 lb

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