Amyloglucosidase (Aspergillus niger)

Item No.  E-AMGDF-40ML

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High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For use in Megazyme Total Starch and Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

E-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.

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Application Examples Applications for the measurement of starch and dietary fiber in the food and feeds industry.
Approvals AOAC Method 2000.11 and GB Standard 5009.245-2016
Brand Megazyme
CAS Number 9032-08-0
CAZy Family GH15
EC Number
Enzyme Amyloglucosidase
Expression From Aspergillus niger
Formulation In 50% (v/v) glycerol
Molecular Weight 143500
Package Dimensions 1.20 IN x 1.20 IN x 3.70 IN
Package Weight 0.18 LB
pH Optima 4.0-4.4
Physical Form Solution
Source Aspergillus niger
Specificity Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins.
Specific Activity ~ 36 U/mg (40°C, pH 4.5 on soluble starch);
~ 200 U/mL (40°C, pH 4.5 on p-nitrophenyl β-maltoside);
~ 3,260 U/mL (40°C, pH 4.5 on soluble starch).
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Synonyms glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase
Temperature Optima (°C) 70
Unit Definition One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40°C.