Amyloglucosidase (Aspergillus niger)

SKU No.  700004182  |  Catalog No.  E-AMGDF-40ML

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High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For use in Megazyme Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

E-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.

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Specifications
BrandMegazyme
ApprovalsAOAC Method 2000.11 and GB Standard 5009.245-2016
EC Number3.2.1.3
EnzymeAmyloglucosidase
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 1 year under recommended storage conditions

Application ExamplesApplications for the measurement of starch and dietary fiber in the food and feeds industry.
FormulationIn 50% (v/v) glycerol
Physical FormSolution
CAS Number9032-08-0
CAZy FamilyGH15
Synonymsglucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase
SourceAspergillus niger
Molecular Weight143500
ExpressionFrom Aspergillus niger
SpecificityHydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins.
Specific Activity~ 200 U/mL (40°C, pH 4.5 on p-nitrophenyl β-maltoside);
~ 3,260 U/mL (40°C, pH 4.5 on soluble starch).
Unit DefinitionOne Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40°C.
Temperature Optima (°C)70
pH Optima4
Package Dimensions1.20 in x 1.20 in x 3.70 in
Package Weight0.18 lb

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