Megazyme® Amyloglucosidase (Aspergillus niger)
SKU No. 700004182 | Catalog No. E-AMGDF-40ML
AOAC OMA
High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and analytical testing applications.
For use in Megazyme Dietary Fiber methods. Amyloglucosidase, Fructanase (E-FRMXPD) and Isoamylase (E-ISAMY) are also utilised in the enzyme hydrolysis step of validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods.
E-AMGDF-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
| Specifications | ||
|---|---|---|
| Brand | Megazyme® | |
| Approvals | For use with AOAC OMA 2000.11 | |
| EC Number | 3.2.1.3 | |
| Storage Conditions | Storage Temperature: 2-8°C Stability: > 1 year under recommended storage conditions | |
| Application Examples | Applications for the measurement of starch and dietary fiber in the food and feeds industry. | |
| Formulation | In 50% (v/v) glycerol | |
| Physical Form | Solution | |
| CAS Number | 9032-08-0 | |
| Synonyms | glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase | |
| Source | Aspergillus niger | |
| Molecular Weight | 143500 | |
| Expression | From Aspergillus niger | |
| Specificity | Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins. | |
| Specific Activity | ~ 200 U/mL (40°C, pH 4.5 on p-nitrophenyl β-maltoside); | |
| Unit Definition | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40°C. | |
| Temperature Optima (°C) | 70 | |
| Package Dimensions | 1.20 in x 1.20 in x 3.70 in | |
| Package Weight | 0.18 lb | |
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