Amyloglucosidase (Aspergillus niger) Glycerol Free
Item No. E-AMGDFNG-20ML
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High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Specifications | ||
|---|---|---|
| Brand | Megazyme | |
| EC Number | 3.2.1.3 | |
| Enzyme | Amyloglucosidase | |
| Storage Conditions |
Storage Temperature: 2-8°C Stability: > 4 years at 4°C |
|
| Application Examples | This enzyme is recommended for use in Total Dietary Fiber analytical procedures and the Megazyme Total Starch test method. The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method). | |
| Formulation | In buffered solution | |
| Physical Form | Solution | |
| CAS Number | 9032-08-0 | |
| CAZy Family | GH15 | |
| Synonyms | glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase | |
| Source | Aspergillus niger | |
| Molecular Weight | 143500 | |
| Expression | From Aspergillus niger | |
| Specificity | Hydrolysis of terminal (1,4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose. | |
| Specific Activity |
~ 36 U/mg (40°C, pH 4.5 on soluble starch); ~ 400 U/mL on p-nitrophenyl β-maltoside; ~ 6,520 U/mL on soluble starch. |
|
| Unit Definition | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4.5 at 40°C. | |
| Temperature Optima (°C) | 70 | |
| pH Optima | 4.0-4.4 | |
| Package Weight | 0.10 | |