Amyloglucosidase (Aspergillus niger) Glycerol Free

Item No.  E-AMGDFNG-20ML

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Expect availability 02/02/2022
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High purity Amyloglucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Application Examples This enzyme is recommended for use in Total Dietary Fiber analytical procedures and the Megazyme Total Starch test method. The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).
Brand Megazyme
CAS Number 9032-08-0 
CAZy Family GH15
EC Number
Enzyme Amyloglucosidase
Expression From Aspergillus niger
Formulation In buffered solution
Molecular Weight 143500
Package Dimensions 1.10 IN x 1.10 IN x 2.30 IN
Package Weight 0.10 LB
pH Optima 4.0-4.4
Physical Form Solution
Source Aspergillus niger
Specificity Hydrolysis of terminal (1,4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose.
Specific Activity ~ 36 U/mg (40°C, pH 4.5 on soluble starch);
~ 400 U/mL on p-nitrophenyl β-maltoside;
~ 6,520 U/mL on soluble starch.
Storage Conditions

Storage Temperature: 2-8°C

Stability: > 4 years at 4°C

Synonyms glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase
Temperature Optima (°C) 70
Unit Definition One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4.5 at 40°C.