Reveal® Q+ for T-2/HT-2

Item No.  8285

  • Quantitative lateral flow format
  • For use with portable reader with data management software
  • Rapid and accurate quantitative testing
This item has shipping restrictions.
$320.50
In stock
Out of stock
Expect availability 05/12/2021
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Reveal® Q+ for T-2/HT-2 is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format intended for the quantitative testing of T-2/HT-2 in grain and grain products.
Specifications
Analyte T-2/HT-2
Brand Reveal®
Limit of Detection 50 ppb
Package Dimensions 8.30 IN x 5.20 IN x 2.40 IN
Package Weight 0.44 LB
Platform Lateral Flow
Quantity per Package 25 tests
Range of Quantitation 50.00 ppb - 600.00 ppb
Result Type Quantitative
Testing Time 6.00 Minutes

  1. 25 Reveal Q+ for T-2/HT-2 test strips
  2. 25 red sample dilution cups
  3. 25 sample cups
  4. 1 bottle of sample diluent
  5. Instructions for use
  1. Sample collection cups with lids (Neogen® item 9428)
  2. Agri-Grind grinder or equivalent (Neogen item 9401, 9453)
  3. Scale capable of weighing 5–50 g ± 0.1 g (Neogen item 9427)
  4. Timer (Neogen item 9426)
  5. Reveal sample cup rack (Neogen item 9475)
  6. AccuScan reader: AccuScan III (Neogen item 9590), AccuScan Pro (Neogen item 9565), or AccuScan Gold (Neogen item 9595)
  7. Dispensing pump or graduated cylinder (Neogen item 9448, 9447)
  8. Filter syringe (Neogen item 9420)
  9. Sample collection tubes with caps (Neogen item 9421, 9421B)
  10. Pipettor, 100 µL (Neogen item 9272, 9278)
  11. Pipette tips, 100 µL (Neogen item 9407, 9410, 9417)
  12. Pipettor, 500 µL (Neogen item 9336)
  13. Pipette tips, 200–1000 µL (Neogen item 9464, 9487)

Technology

Assay Principle

Reveal Q+ for T-2/HT-2 is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for T-2/HT-2 toxins conjugated to colloidal gold particles. If T-2/HT-2 toxins are present, they will be captured by the particle-antibody complex. The T-2/HT-2 toxins-antibody-particle complex then is wicked onto a membrane, which contains a zone of T-2/HT-2 toxins conjugated to a protein carrier. This zone captures any uncomplexed T-2/HT-2 toxins antibody, allowing the particles to concentrate and form a visible line. As the level of T-2/HT-2 toxins in a sample increases, free T-2/HT-2 toxins will complex with the antibody-gold particles. This allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of T-2/HT-2 toxins in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan® reader convert these line densities into a quantitative result displayed in parts per billion (ppb). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of T-2/HT-2 toxins, ensuring the strip is functioning properly.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.


Support

Validation

Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more