Reveal® Q+ for T-2/HT-2

Assay Principle

Reveal Q+ for T-2/HT-2 is a single-step lateral flow immunochromatographic assay based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for T-2/HT-2 toxins conjugated to colloidal gold particles. If T-2/HT-2 toxins are present, they will be captured by the particle-antibody complex. The T-2/HT-2 toxins-antibody-particle complex then is wicked onto a membrane, which contains a zone of T-2/HT-2 toxins conjugated to a protein carrier. This zone captures any uncomplexed T-2/HT-2 toxins antibody, allowing the particles to concentrate and form a visible line. As the level of T-2/HT-2 toxins in a sample increases, free T-2/HT-2 toxins will complex with the antibody-gold particles. This allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of T-2/HT-2 toxins in the sample increases, the test line density decreases. Algorithms programmed into the AccuScan^® reader convert these line densities into a quantitative result displayed in parts per billion (ppb). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of T-2/HT-2 toxins, ensuring the strip is functioning properly.