Reveal® Q+ for Aflatoxin ENDPOINT

Item No.  8075

  • Rapid and accurate quantitative testing
  • For use with the Raptor® Solo Platform
  • Uses the standard mode track
This item has shipping restrictions.
$235.50
In stock
Out of stock
Expect availability 10/20/2021
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Reveal® Q+ for Aflatoxin ENDPOINT is a lateral flow immunochromatographic assay based on a competitive immunoassay format intended for the quantitative testing of aflatoxin in corn using the Raptor® Solo Platform.

Aflatoxin is a toxic and carcinogenic substance produced by certain strains of the molds Aspergillus flavus and A. parasiticus. There are four principle types of aflatoxin: B1, B2, G1 and G2. Aflatoxin B1 is the most frequently encountered of the group and the most toxic. The commodities most affected by aflatoxin are corn, peanuts, cottonseed, milo, and the majority of tree nuts.

The effects on animals that ingest excessive amounts of the toxin range from chronic health and performance problems to death. Aflatoxin has been shown to cause liver damage or cancer, decreased milk and egg production, immune suppression, and interference with reproductive efficiency.

Many countries have set maximum allowable levels of aflatoxin in food and feed. Therefore, accurate determination of the toxin’s presence is crucially important to those monitoring the quality of food and feed in which aflatoxin may occur. Testing these commodities for the toxin requires careful sampling, chemical extraction, sanitation, and quantitative analysis.

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Specifications
Analyte Aflatoxin
Brand Reveal®
Cross Reactivity Total aflatoxins (B1, B2, G1, G2)
Limit of Detection 2 ppb
Platform Lateral Flow
Quantity per Package 25 tests
Range of Quantitation 3.00 ppb - 100.00 ppb
Result Type Quantitative
Storage Conditions Room Temperature (18–30°C, 64–86°F)
Testing Time 6.00 Minutes

  • 25 Reveal Q+ for Aflatoxin test strips
  • 25 red sample dilution cups
  • 25 clear sample cups
  • 1 bottle of sample diluent
  • Instructions for use
  1. Extraction Materials
    • 65% ethanol solution (NEOGEN item 8073, 8074)
    • Sample collection cups with lids 125 mL (NEOGEN item 9428, 9428B)
    • Sample collection tubes with caps (NEOGEN item 9421, 9421B)
    • Filter syringes (NEOGEN item 9272, 9860) OR
    • Centrifuge, mini (NEOGEN item 9330)
    • Micro-centrifuge tubes (NEOGEN item 9172)
    • Dispensing pump or graduated cylinder (NEOGEN item 9448, 9447)
    • Agri-Grind grinder or equivalent (NEOGEN item 9401, 9453)
    • Scale capable of weighing 5–50 g ± 0.1 g (NEOGEN item 9427)
    • Timer (NEOGEN item 9426, 9452)
    • Reveal sample cup rack (NEOGEN item 9475)
    • Raptor Solo Integrated Analysis Platform (NEOGEN item 9696)
    • Pipettor, 100 μL (NEOGEN item 9272, 9860)
    • Pipette tips, 100 μL (NEOGEN item 9407, 9410, 9417)
    • Pipettor, 500 μL (NEOGEN item 9336, 9291)
    • Pipette tips, 200–1000 μL (NEOGEN item 9464, 9487, 9293, 9292)

    Technology

    In the test, the extract is wicked through the reagent zone, which contains antibodies specific for aflatoxin conjugated to colloidal gold particles (gold complex). If aflatoxin is present, it will be captured by the gold complex. The gold complex, along with any free gold-complex, is then wicked onto a membrane, which contains a zone of aflatoxin conjugated to a protein carrier. This zone captures any unbound gold complex, allowing the particles to concentrate and form a visible line. As the level of aflatoxin in a sample increases, free aflatoxin will bind with the gold complex allowing the less gold complex to be captured in the test zones. Therefore, as the concentration of aflatoxin in the sample increases, the test line density decreases.

    Algorithms programmed into the readers convert the line intensities into a quantitative result displayed in parts per billion (ppb). The membrane also contains a control zone where an immune complex present in the reagent zone is captured by an antibody, forming a visible line. The control line will always form regardless of the presence of aflatoxin, ensuring the strip is functioning properly.

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    Training

    Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.