NeoColumn™ for Zearalenone

Item No.  8140

  • High performance immunoaffinity column
  • Unique leak-proof stopper with "wings" for easy removal
  • State of the art beaded gel bed for maximum consistency of performance
  • Performance exceeds current EU regulatory requirements
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$865.50
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Expect availability in 2-4 weeks
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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
NeoColumn™ for Zearalenone is a high performance immunoaffinity column designed for clean-up and concentration of sample prior to running on an HPLC system or Veratox®. NeoColumn is suitable for use in the determination of zearalenone in a wide range of commodities such as maize, wheat, animal feed, maize gluten and breakfast cereal.
Specifications
Analyte Zearalenone
Brand NeoColumn™
Limit of Detection 5 ppb
Package Dimensions 5.30 IN x 10.40 IN x 3.80 IN
Package Weight 1.00 LB
Platform Immunoaffinity
Quantity per Package 50 columns
Result Type Quantitative

NeoColumn for Zearalenone WB (Neogen® item 8140)
  1. Scale, electronic (digital) (Neogen item 9427)
  2. Glassware/plasticware
  3. Laboratory blender (Neogen item 9493)
  4. Centrifuge or filter paper/filter syringe (Neogen item 9429, 9420)
  5. Extraction solvents (HPLC grade)
  6. Distilled water (HPLC grade)
  7. Phosphate Buffered Saline (PBS) (Neogen item 8425)
  8. Clamp stand (Neogen item 9358)
  9. Column rack (Neogen item 9359)
  10. Pipettes and tips (Neogen items 9463, 9464 and 9487)
  11. Glass or resin–treated (Sigmacote or equivalent) plastic syringe barrel (10 mL and pump unit) (Neogen item 9365)
  12. Syringe adapter (Neogen item 9371)
  13. pH meter
  14. Zearalenone standards (see page 5)
  15. Sodium hypochlorite solution/tablets
  16. Methanol (HPLC grade needed for elution. ACS grade may be used for extraction only.)
  17. Whatman no. 4 filters (Neogen item 9429)
  18. Whatman GF/A filters (Neogen item 9358)
  19. Acetonitrile (For HPLC testing only)

Technology

Zearalenone is extracted from a ground sample by blending and filtering. Extracted toxin in the filtrate is sampled and diluted with Phosphate Buffered Saline (PBS). The diluted extract is filtered and applied to the column. Positive pressure is used to induce flow through the column allowing the antibody to capture any zearalenone present. Then, the column is washed to remove any unbound materials. Bound zearalenone is eluted using HPLC grade methanol and is detected by HPLC or Veratox® enzyme-linked immunosorbent assay (ELISA) methods.

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Validation

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