NeoColumn™ for T-2/HT-2

Item No.  8240

  • High performance immunoaffinity column
  • Unique leak-proof stopper with "wings" for easy removal
  • State of the art beaded gel bed for maximum consistency of performance
  • Custom isocratic HPLC assay - highly sensitive limit of quantification
  • Superior protocol developed for the occasional or expert user
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Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
NeoColumn™ for T-2/HT-2 is a high performance immunoaffinity column designed for clean-up and concentration of sample prior to running on an HPLC system or Veratox®. Neocolumn is suitable for use in the determination of T-2 and HT-2 in a wide range of commodities such as baby food, oats, maize, wheat, barley and animal feed.
Analyte T-2/HT-2
Brand NeoColumn™
Limit of Detection 12.5 ppb T-2 or HT-2 toxins
Platform Immunoaffinity
Quantity per Package 50 columns
Range of Detection 12.50 ppb - 1,000.00 ppb
Result Type Quantitative

NeoColumn for T-2/HT-2 WB (Neogen item 8240)
  1. Scale, electronic (digital) (Neogen item 9427)
  2. Glassware/plasticware
  3. Laboratory blender (Neogen item 9493)
  4. Centrifuge or filter paper/filter syringe (Neogen items 9429, 9420)
  5. Extraction solvents (HPLC grade)
  6. Distilled or deionized water (HPLC grade)
  7. Phosphate Buffered Saline (PBS) with Tween or Neogen wash buffer (Neogen item 8428)
  8. Clamp stand (Neogen item 9358)
  9. Column rack (Neogen item 9359)
  10. Pipettes and tips (Neogen items 9276, 9410 and 9407)
  11. Glass or resin – treated (Sigmacote or equivalent) plastic syringe barrel (10 mL and pump unit) (Neogen item 9365)
  12. Syringe adapter (Neogen item 9371)
  13. pH meter
  14. Sodium hypochlorite solution/tablets
  15. Methanol (HPLC grade needed for elution. ACS grade may be used for extraction only.)
  16. Whatman no. 4 filters (Neogen item 9429)
  17. Whatman GF/A filters (Neogen item 9352)
  18. 1-Anthroylcyanide (1-An), 100 mg (Wako Chemicals 017-12101)
  19. 4-Dimethylaminopyridine reagent plus (99 % (DMAP) (Aldrich, 10,770)
  20. Toulene ACS reagent (Sigma T4428)
  21. T-2 toxin (Sigma T4887)
  22. HT-2 toxin (Sigma T4138)
  23. Hot plate, capable of reaching 50°C
  24. Mini-vap evaporator (Sigma 22971)
  25. Nitrogen
  26. Acetonitrile (For HPLC testing only)
  27. NaCl (Neogen item 7725G)


T-2/HT-2 toxins are extracted from a ground sample by blending and filtering. Extracted toxins in the filtrate are sampled and diluted with water. The diluted extract is filtered and applied to the column. Positive pressure is used to induce flow through the column allowing the antibody to capture any T-2/HT-2 toxins present. Then, the column is washed to remove any unbound materials. Bound T-2/HT-2 toxins are eluted using HPLC grade methanol and are detected by HPLC or Veratox® enzyme-linked immunosorbent assay (ELISA) methods



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