NeoColumn™ for DON

Item No.  8340

  • High performance immunoaffinity column
  • Unique leak-proof stopper with "wings" for easy removal
  • State of the art beaded gel bed for maximum consistency of performance
  • Performance exceeds current EU and CEN regulatory requirements
  • Superior protocol developed for the occasional or expet user
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$865.50
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Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
NeoColumn™ for DON is a high performance immunoaffinity column designed for clean-up and concentration of sample prior to running on an HPLC system or Veratox®. NeoColumn is suitable for use in the determination of DON in a wide range of commodities such as maize, wheat, durum wheat, malted barley, beer and animal feed.
Specifications
Analyte Deoxynivalenol (DON)
Brand NeoColumn™
Limit of Detection 0.1 - 4 ppm
Platform Immunoaffinity
Quantity per Package 50 columns
Result Type Quantitative

NeoColumn for DON WB (Neogen® item 8340)
  1. Scale, electronic (digital) (Neogen item 9427)
  2. Glassware/plasticware
  3. Laboratory blender (Neogen item 9493)
  4. Centrifuge or filter paper/filter syringe (Neogen item 9429, 9420)
  5. Extraction solvents (HPLC grade)
  6. Distilled or deionized water (HPLC grade)
  7. Pipettes and tips (Neogen items 9463, 9464 and 9487)
  8. Clamp stand (Neogen item 9358)
  9. Column rack (Neogen item 9359)
  10. Glass or resin – treated (Sigmacote or equivalent) plastic syringe barrel (10 mL and pump unit) (Neogen item 9365)
  11. Certified DON standards (see page 5)
  12. pH meter (for beer analysis)
  13. Fume hood
  14. Hot plate capable of reaching 60°C
  15. Mini-Vap evaporator (Sigma 22971)
  16. Nitrogen
  17. Syringe adapter for NeoColumn (Neogen item 9371)
  18. Sodium hypochlorite solution/tablets
  19. Methanol (HPLC grade needed for elution)
  20. Whatman no. 4 filters (Neogen item 9429)
  21. Whatman 2V and GF/A filters (Neogen items 9358)
  22. Acentonitrile (For HPLC testing only)

Technology

DON is extracted from a ground sample by blending and filtering. Extracted toxin in the filtrate is sampled and diluted with water. The diluted extract is filtered and applied to the column. Positive pressure is used to induce flow through the column allowing the antibody to capture any DON present. Then, the column is washed to remove any unbound materials. Bound DON is eluted using 100% methanol and is detected by HPLC or Veratox® enzyme-linked immunosorbent assay (ELISA) methods.

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Validation

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