Yersinia Selective Agar (Schiemann's CIN Agar)

Item No.  NCM0182A

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Yersinia Selective Agar is used with cefsulodin and novobiocin for the isolation of Yersinia enterocolitica in a laboratory setting. Yersinia Selective Agar is not intended for use in the diagnosis of disease or other conditions in humans.

This medium is based on the work of Schiemann. It is used for the isolation and enumeration of Yersinia spp. from clinical samples and from food. The selective components are sodium desoxycholate, crystal violet, cefsulodin, irgasan and novobiocin. Yersiniae ferment mannitol with an intense, localized, acid production in the center of the colony which produces a red ‘bull’s eye’ appearance. The ratio of transparent border to red centre varies with serotype and environmental strains may appear rough with an irregular edge. Most other enteric bacteria, if they grow, produce a larger colony with a diffuse pinkish center and opaque outer zone.

Specifications
Brand Neogen® Culture Media
Analyte Yersinia spp.
Platform Dehydrated Culture Media
Mass 500 g
Formula
Formula Liter
Peptone Mix 22.5 g/L
Mannitol 20.0 g/L
Sodium Chloride 1.0 g/L
Magnesium Sulphate 0.1 g/L
Sodium Pyruvate 2.0 g/L
Sodium Deoxycholate 0.5 g/L
Neutral red 0.03 g/L
Crystal Violet 0.001 g/L
Agar 12.0 g/L
Grams per Liter 58.00
Liters per 500 g 9.00
Plates per 500 g 470.00
Preparation
  1. Suspend 58 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool to 45-50ºC and aseptically add 2 vials of C.I.N. Supplement X120.
Previous Item Number Product was previously known as Acumedia Product: 7257 Yersinia Selective Agar; and LabM Product: LAB120 Yersinia C.I.N. Agar Base
Package Dimensions 4.60 in x 2.90 in x 8.60 in
Package Weight 1.35 lb

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