WL Nutrient Agar

Item No.  NCM0118A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

W-L Nutrient Agar is used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations. W-L Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans.

W-L Nutrient Medium was developed by Green and Gray while studying various fermentation processes. An exhaustive study examining methods of fermentation control procedures in worts, beers, liquid yeasts and similar fermentation products led to the development of W-L Nutrient Medium. At a pH of 5.5, counts of viable baker’s yeast will grow on W-L Nutrient Medium. W-L Nutrient Medium is also referred to as “Wallerstein Laboratory Medium”.

Analyte Pitching Yeasts, Yeast & Mold
Brand NEOGEN® Culture Media
Formula Liter
Yeast Extract 4.0 g/L
Enzymatic Digest of Casein 5.0 g/L
Dextrose 50.0 g/L
Monopotassium Phosphate 0.55 g/L
Potassium Chloride 0.425 g/L
Calcium Chloride 0.125 g/L
Magnesium Sulfate 0.125 g/L
Ferric Chloride 0.0025 g/L
Manganese Sulfate 0.0025 g/L
Bromocresol Green 0.022 g/L
Agar 20.0 g/L
Grams per Litre 75.00
Litres per 500g 7.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 360.00
  1. Suspend 80 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC.
Previous Item Number Product was previously known as Acumedia Product: 7488 WL Nutrient Agar; and LabM Product: LAB079 WL Nutrient Agar



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