Vogel Johnson Agar

Item No.  NCM0282A

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$135.00
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$12,226.00
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This item must be ordered in multiples of one case. Quantity per case: 1

Vogel Johnson Agar is used for the isolation of staphylococci in a laboratory setting. Vogel Johnson Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Coagulase-positive staphylococci, primarily Staphylococcus aureus, are among the microorganisms that cause spoilage or chemical changes in cosmetic products. To isolate coagulase-positive, mannitol fermenting staphylococci, Vogel and Johnson modified Tellurite-Glycine Agar by Zebovitz, Evans, and Niven. The modification included increasing mannitol and adding a pH indicator. Vogel Johnson Agar selects and differentiates coagulase-positive staphylococci that ferment mannitol and reduce tellurite.

Vogel Johnson (VJ) Agar is specified in standard methods testing for cosmetics and nutritional supplements.

Specifications
Analyte Staphylococcus
Brand NEOGEN® Culture Media
Formula
Formula Liter
Enzymatic Digest of Casein 10.0 g/L
Yeast Extract 5.0 g/L
Mannitol 10.0 g/L
Dipotassium Phosphate 5.0 g/L
Lithium Chloride 5.0 g/L
Glycine 10.0 g/L
Phenol Red 0.025 g/L
Agar 15.0 g/L
Grams per Litre 60.00
Litres per 500g 8.00
Mass 500 g
Package Dimensions 10.20 IN x 10.20 IN x 11.90 IN
Package Weight 15.30 LB
Plates per 500g 458.00
Preparation
  1. Suspend 60 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC and aseptically add 2 vials (20 mL) of Tellurite Supplement (1%) Chapman (7989). Note: Do not heat medium after Tellurite Supplement has been added.
  5. Mix thoroughly before dispensing.
Previous Item Number Product was previously known as Acumedia Product: 7207 Vogel and Johnson Agar

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