Vogel Johnson Agar
SKU No.  700003656  | Catalog No.  NCM0282A
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Vogel Johnson Agar

500 g

SKU No.  700003656  |  Catalog No.  NCM0282A

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Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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Vogel Johnson Agar is used for the isolation of staphylococci in a laboratory setting. Vogel Johnson Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Coagulase-positive staphylococci, primarily Staphylococcus aureus, are among the microorganisms that cause spoilage or chemical changes in cosmetic products. To isolate coagulase-positive, mannitol fermenting staphylococci, Vogel and Johnson modified Tellurite-Glycine Agar by Zebovitz, Evans, and Niven. The modification included increasing mannitol and adding a pH indicator. Vogel Johnson Agar selects and differentiates coagulase-positive staphylococci that ferment mannitol and reduce tellurite.

Vogel Johnson (VJ) Agar is specified in standard methods testing for cosmetics and nutritional supplements.

Specifications
BrandNeogen® Culture Media
AnalyteStaphylococcus
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein10.0 g/L
Yeast Extract5.0 g/L
Mannitol10.0 g/L
Dipotassium Phosphate5.0 g/L
Lithium Chloride5.0 g/L
Glycine10.0 g/L
Phenol Red0.025 g/L
Agar15.0 g/L
Grams per Liter60.00
Liters per 500 g8.00
Plates per 500 g458.00
Preparation
  1. Suspend 60 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC and aseptically add 2 vials (20 mL) of Tellurite Supplement (1%) Chapman (7989). Note: Do not heat medium after Tellurite Supplement has been added.
  5. Mix thoroughly before dispensing.
Previous Item NumberProduct was previously known as Acumedia Product: 7207 Vogel and Johnson Agar
Package Dimensions10.20 in x 10.20 in x 11.90 in
Package Weight1.00 lb

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