Violet Red Bile Glucose Agar (ISO)

Item No.  NCM0041A

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Expect availability 02/04/2022
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Violet Red Bile Glucose Agar is used for the enumeration of Enterobacteriaceae in foods and is not intended for use in the diagnosis of disease or other conditions in humans. Formulated according to ISO 21528:2017 (all parts), VRBGA is a modification of Violet Red Bile Lactose Agar (NCM0089) introduced by Mossel in 1978. VRBA contains lactose which is fermented by members of the coli/ aerogenes group, this medium gives a ‘coliform’ count. The VRBGA formula modification replaces lactose with glucose. Glucose is fermented by all members of the Enterobacteriaceae thus gives a presumptive Enterobacteriaceae count. Bile salts and crystal violet are used to inhibit Gram positive and non-enteric organisms. The overlay procedure ensures anaerobic conditions and suppresses the growth of non-fermentative Gram negative bacteria.
Analyte Enterobacteriaceae
Brand NEOGEN® Culture Media
Formula Liter
Yeast Extract 3.0 g/L
Enzymatic digest of Animal Tissue 7.0 g/L
Bile Salts 1.5 g/L
Sodium Chloride 5.0 g/L
Glucose 10.0 g/L
Agar 12.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.002 g/L
Grams per Litre 38.50
Litres per 500g 13.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 680.00
  1. Suspend 38.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Cool to 45-50°C.
Previous Item Number Product was previously known as Acumedia Product: 7425 Violet Red Bile Glucose Agar; and LabM Product: LAB088 Violet Red Bile Glucose Agar (VRBGA)



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