Violet Red Bile Agar (VRBA)

Item No.  NCM0025A

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$104.48 each
$992.51 each
$1,943.24 each
$9,507.23 each
In stock
Out of stock
Expect availability 10/07/2022
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Violet Red Bile Agar is used for the enumeration of coliforms in food and dairy products in a laboratory setting. Violet Red Bile Agar is not intended for use in the diagnosis of disease or other conditions in humans and conforms to American Public Health Association (APHA).

The coliform group of bacteria includes aerobic and facultative anaerobic, gram-negative, non-spore forming bacilli. Coliforms ferment lactose and form acid and gas at 35°C within 48 hours. Members of Enterobacteriaceae comprise the majority of the group, but other lactose fermenting organisms may also be included.

Procedures to detect, enumerate, and presumptively identify coliforms are used in testing foods and dairy products. One method for performing the presumptive test for coliforms uses Violet Red Bile Agar, (VRBA). If typical coliform colonies appear, they are tested further to confirm their identification as coliforms. Coliform colonies lower the pH of the medium, subsequently causing their colonies to look red (Neutral Red Dye) and to precipitate the bile salts.

Brand Neogen® Culture Media
Analyte Coliforms, E. coli
Platform Dehydrated Culture Media
Mass 500 g
Formula Liter
Yeast Extract 3.0 g/L
Enzymatic Digest of Gelatin 7.0 g/L
Bile Salts 1.5 g/L
Lactose 10.0 g/L
Sodium Chloride 5.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.002 g/L
Agar 15.0 g/L
Grams per Liter 41.50
Liters per 500 g 12.00
Plates per 500 g 660.00
  1. Suspend 41.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for two minutes. DO NOT AUTOCLAVE.
  3. Cool the medium to 45-50ºC and dispense 15-20 mL into 100 mm petri dishes containing inoculum.
  4. After solidification of the inoculated medium, evenly add a cover layer of 4 mL of the cooled (45-50ºC) agar medium.
Previous Item Number Product was previously known as Acumedia Product: 7165 Violet Red Bile Agar
Package Weight 1.35 lb



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