Violet Red Bile Agar with MUG (VRBA-MUG)

SKU No.  700003150  |  Catalog No.  NCM0064B

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Minimum quantity required: 1
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Violet Red Bile Agar with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli. Violet Red Bile Agar with MUG is not intended for use in the diagnosis of disease or other conditions in humans.

Violet Red Bile Agar W/ MUG is specified in many procedures to enumerate coliforms in food and dairy products. In 1982, Feng and Hartman developed a rapid fluorogenic assay for Escherichia coli by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG) into Lauryl Tryptose Broth. Similarly, incorporating MUG into Violet Red Bile Agar permits the detection of E. coli among the coliform colonies. Standard methods procedures specify Violet Red Bile Agar with MUG for detecting E. coli in food and dairy products by fluorescence. This medium is often abbreviated as VRBA w/ MUG.

Specifications
BrandNeogen® Culture Media
AnalyteColiforms, E. coli
PlatformDehydrated Culture Media
Mass5 kg
Formula
FormulaLiter
Enzymatic Digest of Gelatin7.0 g/L
Yeast Extract3.0 g/L
Bile Salts Mixture1.5 g/L
Lactose10.0 g/L
Sodium Chloride5.0 g/L
Neutral Red0.03 g/L
Crystal Violet0.002 g/L
4-Methylumbelliferyl-β-D-Glucuronide0.1 g/L
Agar15.0 g/L
Grams per Liter41.60
Liters per 500 g13.00
Plates per 500 g710.00
Preparation
  1. Suspend 41.6 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium. DO NOT AUTOCLAVE.
  3. Cool to 45-50ºC.
Previous Item NumberProduct was previously known as Acumedia Product: 7359 Violet Red Bile Agar with MUG (VRBA-MUG)
Package Dimensions11.50 in x 11.50 in x 10.50 in
Package Weight14.00 lb

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