UVM Broth
SKU No.  700002995  | Catalog No.  NCM0012A
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UVM Broth

SKU No.  700002995  |  Catalog No.  NCM0012A

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UVM Broth is used for the selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans. UVM Broth is a modification of the formula described by Donnelly and Baigent. This formula is used for the selective enrichment of Listeria spp. from food samples. Listeria spp. grow over a pH range of 5.0 - 9.6 and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation. Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Beef Extract, and Yeast Extract provide nitrogen, vitamins, and minerals in UVM Broth. The Phosphates are the buffering agents, Sodium Chloride maintains osmotic balance. Nalidixic Acid inhibits growth of Gram-negative organisms. Acriflavin inhibits Gram-positive bacteria. Esculin is hydrolyzed by Listeria spp. The high salt tolerance of Listeria is used as a means to inhibit growth of enterococci.
Specifications
BrandNeogen® Culture Media
AnalyteListeria spp.
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein5.0 g/L
Enzymatic Digest of Animal Tissue5.0 g/L
Beef Extract5.0 g/L
Yeast Extract5.0 g/L
Sodium Chloride20.0 g/L
Disodium Hydrogen Phosphate9.6 g/L
Potassium Dihydrogen Phosphate1.35 g/L
Esculin1.0 g/L
Acriflavin0.012 g/L
Nalidixic Acid0.02 g/L
Grams per Liter52.00
Liters per 500 g10.00
Preparation
  1. Dissolve 52 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
Previous Item NumberProduct was previously known as Acumedia Product: 7409 UVM Modified Listeria Enrichment Broth; and LabM Product: LAB155 UVM Broth Base
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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UVM Broth - Technical Specifications Sheet - English - US

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