Urea Broth Base (Christensen)

Item No.  NCM0177A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
Urea Broth Base is for the detection of urease production, and is not intended for use in the diagnosis of disease or other conditions in humans. This is a liquid version of Christensen’s medium (NCM0180) introduced by Maslen in 1952. This modification allows inoculation by Pasteur pipette, and it is easier to detect contamination in a fluid rather than in a slope. Maslen also claimed that it is easier to detect positive results.
Analyte Proteus
Brand NEOGEN® Culture Media
Formula Liter
Peptone 1.0 g/L
Glucose 1.0 g/L
Disodium Phosphate 1.2 g/L
Potassium Dihydrogen Phosphate 0.8 g/L
Sodium Chloride 5.0 g/L
Phenol Red 0.004 g/L
Grams per Litre 9.00
Litres per 500g 56.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 0.9 grams of the medium in 95ml of purified water.
  2. Heat with frequent agitation to completely dissolve the medium if necessary.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C then add aseptically 5ml of X130/X135 sterile urea solution.
Previous Item Number Product was previously known as LabM Product: LAB131 Urea Broth Base



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