Urea Agar Base (Christensen)

Item No.  NCM0180A

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Urea Agar Base is used with agar for the differentiation of microorganisms on the basis of urease production in a laboratory setting. Urea Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Christensen devised a urea agar medium containing peptone and dextrose that had a reduced buffer content. The medium supported the vigorous growth of many Gram-negative, enteric bacilli and readily permitted observation of urease production. Urea Agar Base may be used as a screening medium (along with Triple Sugar Iron Agar) for the selection of Salmonella and Shigella cultures for serologic classification. Urea Agar Base is also used to detect production of urease by yeast. Urease production is an important differential test in microbiology and outlined in standard methods.

Specifications
Analyte Non-selective
Brand NEOGEN® Culture Media
Formula
Formula Liter
Enzymatic Digest of Gelatin 1.0 g/L
Dextrose 1.0 g/L
Sodium Chloride 5.0 g/L
Monopotassium Phosphate 2.0 g/L
Urea 20.0 g/L
Phenol Red 0.012 g/L
Grams per Litre 21.00
Litres per 500g 24.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 1,300.00
Preparation
  1. Suspend 29 g of the medium in 100 mL of purified water until dissolved completely. Filter sterilize.
  2. Suspend 15 g of Agar No. 1 Bacteriological (NCM0236) in 900 mL of purified water.
  3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Autoclave at 121ºC for 15 minutes.
  5. Cool sterilized agar to 45-50ºC and aseptically add the sterile Urea Agar Base.
  6. Mix thoroughly and dispense into sterile tubes. Place tubes in a slanted position.
Previous Item Number Product was previously known as Acumedia Product: 7226 Urea Agar Base; and LabM Product: LAB130 Urea Agar Base

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