Tryptic Soy Broth, Modified with Acid Digest of Casein

Item No.  NCM0026A

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Minimum quantity required: 1
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Tryptic Soy Broth, Modified with Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. Tryptic Soy Broth, Modified with Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. This medium conforms to USDA Formulation with Acid Digest of Casein.

The first major outbreak of E. coli O157:H7 was in 1982 and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.

Specifications
Analyte Enterohemorrhagic, E. coli, E. coli (EHEC)
Brand NEOGEN® Culture Media
Formula
Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Acid Digest of Casein 10.0 g/L
Sodium Chloride 5.0 g/L
Dipotassium Phosphate 4.0 g/L
Enzymatic Digest of Soybean Meal 3.0 g/L
Dextrose 2.5 g/L
Bile Salts No. 3 1.5 g/L
Grams per Litre 43.00
Litres per 500g 12.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Preparation
  1. Dissolve 43 grams of the medium in 1 Liter of purified water.
  2. Autoclave at 121°C for 15 minutes.
  3. If required, cool to 45-50°C and add 4 mL of NCM4040-0.5*, Novobiocin Supplement reconstituted using 5 mL sterile deionized/RO water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as Acumedia Product: 7167 Tryptic Soy Broth, Modified with Acid Digest of Casein

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