Tryptic Soy Broth, Modified with Acid Digest of Casein
SKU No.  700003036  | Catalog No.  NCM0026A
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Tryptic Soy Broth, Modified with Acid Digest of Casein

SKU No.  700003036  |  Catalog No.  NCM0026A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
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Tryptic Soy Broth, Modified with Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. Tryptic Soy Broth, Modified with Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. This medium conforms to USDA Formulation with Acid Digest of Casein.

The first major outbreak of E. coli O157:H7 was in 1982 and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.

Specifications
BrandNeogen® Culture Media
AnalyteEnterohemorrhagic, E. coli, E. coli (EHEC)
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Enzymatic Digest of Casein17.0 g/L
Acid Digest of Casein10.0 g/L
Sodium Chloride5.0 g/L
Dipotassium Phosphate4.0 g/L
Enzymatic Digest of Soybean Meal3.0 g/L
Dextrose2.5 g/L
Bile Salts No. 31.5 g/L
Grams per Liter43.00
Liters per 500 g12.00
Preparation
  1. Dissolve 43 grams of the medium in 1 Liter of purified water.
  2. Autoclave at 121°C for 15 minutes.
  3. If required, cool to 45-50°C and add 4 mL of NCM4040-0.5*, Novobiocin Supplement reconstituted using 5 mL sterile deionized/RO water.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item NumberProduct was previously known as Acumedia Product: 7167 Tryptic Soy Broth, Modified with Acid Digest of Casein
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

Documents

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Tryptic Soy Broth, Modified with Acid Digest of Casein - Technical Specifications Sheet - English - US

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