Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest Casein

Item No.  NCM0137A

This item has shipping restrictions.
In stock
Out of stock
Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

TSB, Modified with 20mg Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. TSB Modified w/20 mg Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans.

The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. TSB, Modified w/20 mg Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing. This formulation conforms to the USDA/FSIS Microbiology Laboratory Guidebook.

Analyte E. coli, E. coli (EHEC), Enterohemorrhagic
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Acid Digest of Casein 10.0 g/L
Sodium Chloride 5.0 g/L
Dipotassium Phosphate 4.0 g/L
Enzymatic Digest of Soybean Mea 3.0 g/L
Dextrose 2.5 g/L
Bile Salts No. 3 1.5 g/L
Novobiocin 0.020 g/L
Grams per Litre 43.02
Litres per 500g 12.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 43.02 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7731 Tryptic Soy Broth, Modified with 20 mg/L Novobiocin & Acid Digest of Casein



Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more