Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest Casein

Item No.  NCM0137A

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$139.00
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$12,524.00
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TSB, Modified with 20mg Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. TSB Modified w/20 mg Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans.

The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. TSB, Modified w/20 mg Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing. This formulation conforms to the USDA/FSIS Microbiology Laboratory Guidebook.

Specifications
Analyte E. coli, E. coli (EHEC), Enterohemorrhagic
Brand NEOGEN® Culture Media
Formula
Formula Liter
Enzymatic Digest of Casein 17.0 g/L
Acid Digest of Casein 10.0 g/L
Sodium Chloride 5.0 g/L
Dipotassium Phosphate 4.0 g/L
Enzymatic Digest of Soybean Mea 3.0 g/L
Dextrose 2.5 g/L
Bile Salts No. 3 1.5 g/L
Novobiocin 0.020 g/L
Grams per Litre 43.02
Litres per 500g 12.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Preparation
  1. Dissolve 43.02 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7731 Tryptic Soy Broth, Modified with 20 mg/L Novobiocin & Acid Digest of Casein

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