Tryptic Soy Blood Agar Base No.2

Item No.  NCM0098A

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Tryptic Soy Blood Agar Base No. 2 is used with blood for the isolation and cultivation of a wide variety of microorganisms in a laboratory setting. Tryptic Soy Blood Agar Base No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.

Blood Agar Bases are typically supplemented with 5 - 10% sheep, rabbit, or horse blood, and used for isolating, cultivating, and determining hemolytic reactions of fastidious pathogenic microorganisms. Without enrichment, this blood agar base can be used as a general purpose medium.

Tryptic Soy Agar is based on the Soybean-Casein Digest formula specified by US Pharmacopeia. Tryptic Soy Agar is a nutritious base, and a variety of supplements, including blood, are often added to enhance this medium. Tryptic Soy Blood Agar Base No. 2 was formulated to achieve good growth and improve hemolytic reactions of important pathogenic bacteria. Blood Agar Base media are specified in standard methods for food testing.

Analyte Non-selective
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 15.0 g/L
Enzymatic Digest of Soybean Meal 5.0 g/L
Sodium Chloride 5.0 g/L
Agar 15.0 g/L
Grams per Litre 40.00
Litres per 500g 13.00
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 680.00
  1. Suspend 40 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cooled to 45 - 50°C.
  5. Prepare 5 - 10% blood agar by aseptically adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium.
Previous Item Number Product was previously known as Acumedia Product: 7654 Tryptic Soy Blood Agar Base No. 2