Triple Sugar Iron (TSI) Agar

Item No.  NCM0144A

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$90.83 each
$862.84 each
$1,689.35 each
$8,265.08 each
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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Triple Sugar Iron (TSI) Agar is used for the differentiation of microorganisms on the basis of dextrose, lactose, and sucrose fermentation and hydrogen sulfide production. Triple Sugar Iron (TSI) Agar is not intended for use in the diagnosis of disease or other conditions in humans.

This is a modification of the Krumwiede and Kohn medium of 1917 which differentiates some of the Enterobacteriaceae on the basis of four reactions; fermentation of lactose, glucose and sucrose and H2S production. This medium should be used in conjunction with a urease test to eliminate Proteus spp. when screening for Salmonella spp.

Specifications
Brand Neogen® Culture Media
Analyte Enterobacteriaceae
Platform Dehydrated Culture Media
Mass 500 g
Formula
Formula Liter
Beef Extract 3.0 g/L
Yeast Extract 3.0 g/L
Peptone Mixture 20.0 g/L
Sodium Chloride 5.0 g/L
Lactose 10.0 g/L
Sucrose 10.0 g/L
Glucose 1.0 g/L
Ferric Citrate 0.3 g/L
Sodium Thiosulphate 0.3 g/L
Phenol Red 0.025 g/L
Agar 12.0 g/L
Grams per Liter 65.00
Liters per 500 g 8.00
Plates per 500 g 420.00
Preparation
  1. Suspend 65 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Dispense into tubes
  4. Autoclave at 121ºC for 15 minutes.
  5. Allow to set as a slope ensuring that the slant is over a butt approximately 3 cm deep.
Previous Item Number Product was previously known as Acumedia Product: 7162 Triple Sugar Iron (TSI) Agar; and LabM Product: LAB053 Triple Sugar Iron (TSI) Agar
Package Weight 1.35 lb

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