Triple Sugar Iron (TSI) Agar

Item No.  NCM0144A

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Expect availability 01/26/2022
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Triple Sugar Iron (TSI) Agar is used for the differentiation of microorganisms on the basis of dextrose, lactose, and sucrose fermentation and hydrogen sulfide production. Triple Sugar Iron (TSI) Agar is not intended for use in the diagnosis of disease or other conditions in humans.

This is a modification of the Krumwiede and Kohn medium of 1917 which differentiates some of the Enterobacteriaceae on the basis of four reactions; fermentation of lactose, glucose and sucrose and H2S production. This medium should be used in conjunction with a urease test to eliminate Proteus spp. when screening for Salmonella spp.

Analyte Enterobacteriaceae
Brand NEOGEN® Culture Media
Formula Liter
Beef Extract 3.0 g/L
Yeast Extract 3.0 g/L
Peptone Mixture 20.0 g/L
Sodium Chloride 5.0 g/L
Lactose 10.0 g/L
Sucrose 10.0 g/L
Glucose 1.0 g/L
Ferric Citrate 0.3 g/L
Sodium Thiosulphate 0.3 g/L
Phenol Red 0.025 g/L
Agar 12.0 g/L
Grams per Litre 65.00
Litres per 500g 8.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 420.00
  1. Suspend 65 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Dispense into tubes
  4. Autoclave at 121ºC for 15 minutes.
  5. Allow to set as a slope ensuring that the slant is over a butt approximately 3 cm deep.
Previous Item Number Product was previously known as Acumedia Product: 7162 Triple Sugar Iron (TSI) Agar; and LabM Product: LAB053 Triple Sugar Iron (TSI) Agar



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