Tetrathionate (TT) Broth Base, Hajna

Item No.  NCM0143A

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Tetrathionate (TT) Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella species (except S. typhi) from samples in a laboratory setting. Tetrathionate (TT) Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans.

Tetrathionate Broth Base, Hajna, is recommended in the Compendium of Methods for the Microbiological Examination of Foods and the FDA/BAM for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and whole eggs.. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Numerous reported cases of Salmonella enteritidis (SE) infections are associated with contaminated shell eggs. SE infections can be very serious and symptoms can include diarrhea, fever, abdominal cramps, headache, nausea and vomiting. Eggs can become contaminated on the farm because a laying hen can become infected with SE and pass the bacteria into the egg before it is laid. If the egg is not refrigerated, the bacteria can grow inside the uncracked, whole egg. Tetrathionate Broth Base, Hajna was developed by Hajna and Damon.

Brand Neogen® Culture Media
Analyte Salmonella spp.
Platform Dehydrated Culture Media
Mass 500 g
Formula Liter
Sodium Thiosulfate 38.0 g/L
Calcium Carbonate 25.0 g/L
Peptone Mixture 18.0 g/L
Sodium Chloride 5.0 g/L
D-Mannitol 2.5 g/L
Yeast Extract 2.0 g/L
Glucose 0.5 g/L
Sodium Deoxycholate 0.5 g/L
Brilliant Green 0.01 g/L
Grams per Liter 91.50
Liters per 500 g 5.00
  1. Suspend 91.5 g of the medium in 960 mL of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE. Cool to 45 - 50ºC.
  4. Just prior to inoculation, add 40 mL of Iodine/Potassium Iodide Solution to the prepared Tetrathionate Broth Base, Hajna.
  5. Dispense into sterile capped containers in 10 mL volumes.
  6. Note: Do not add Iodine/Potassium Iodide Solution to tubes until just before inoculation. Tetrathionate inhibits by oxidation of Thiosulfate through the addition of Iodine just prior to use.

Previous Item Number Product was previously known as Acumedia Product: 7740 Tetrathionate (TT) Broth Base, Hajna; and LabM Product: LAB531 Tetrathionate (TT) Broth Base, Hajna
Package Dimensions 4.60 in x 2.90 in x 8.60 in
Package Weight 1.35 lb


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