TAT Broth

Item No.  NCM0091A

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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

TAT Broth is used for the detection of microorganisms in cosmetics and topical drugs in a laboratory setting. TAT Broth is not intended for use in the diagnosis of disease or other conditions in humans.

TAT Broth is prepared for use in the examination of cosmetics and topical drugs. TAT Broth, with the addition of Polysorbate 20, is recommended for sterility testing of viscous materials, including salves or ointments. It is especially adapted for sterility testing of cosmetics. Cosmetic products are subject to contamination during manufacturing and use by consumers. Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeast, and molds. TAT (Tryptone-Azolectin-Tween) Broth is also referred to as Fluid Casein Digest-Soy Lecithin Polysorbate 20 Medium.

Analyte Non-selective
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 20.0 g/L
Lecithin 5.0 g/L
Grams per Litre 25.00
Litres per 500g 20.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Suspend 25 grams of the medium in 960 mL of purified water until evenly dispersed.
  2. At room temperature, add 40 mL of Polysorbate 20 to the suspended medium.
  3. Place the mixture in a 48-50ºC water bath for 30 minutes. Stir occasionally to dissolve.
  4. Autoclave at 121ºC for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7219 TAT Broth