Sorbitol MacConkey Agar

Item No.  NCM0167A

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Expect availability 12/31/2050
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Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1

Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7. Serotype O157 has been implicated in serious foodborne diseases.

MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.

Enzymatic Digest of Gelatin, Enzymatic Digest of Casein, and Enzymatic Digest of Animal Tissue are the nitrogen and vitamin sources in MacConkey Agar W/ Sorbitol. Sorbitol is the fermentable carbohydrate; typically enteropathogenic strains produce colorless colonies. Bile Salts Mixture and Crystal Violet are the selective agents, inhibiting Gram-positive cocci. Sodium Chloride maintains the osmotic environment, and Neutral Red is the pH indicator. Agar is the solidifying agent. The selectivity of the medium can be increased through the addition of Cefixime and tellurite.

Specifications
Analyte E. coli O157
Brand NEOGEN® Culture Media
Formula
Formula Liter
Enzymatic Digest of Gelatin 17.0 g/L
Enzymatic Digest of Casein 1.5 g/L
Enzymatic Digest of Animal Tissue 1.5 g/L
Sorbitol 10.0 g/L
Bile Salts Mixture 1.5 g/L
Sodium Chloride 5.0 g/L
Neutral Red 0.03 g/L
Crystal Violet 0.001 g/L
Agar 13.5 g/L
Grams per Litre 50.00
Litres per 500g 10.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 560.00
Preparation
  1. Suspend 50 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45-50°C. 5. If required add 2 vials of NCM4045-0.5* Cefixime Tellurite Supplement, each reconstituted with 5 mL of sterile/RO water and mix well before dispensing.

*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions.

Previous Item Number Product was previously known as Acumedia Product: 7320 MacConkey Agar with Sorbitol; and LabM Product: LAB161 Sorbitol MacConkey Agar

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