Slanetz and Bartley Agar

Item No.  NCM0197A

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$157.50
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Expect availability 01/31/2022
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Minimum quantity required: 1
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Slanetz and Bartley Medium is used for the enumeration of enterococci from water samples as described in ISO 7899-2, and is not intended for use in the diagnosis of disease or other conditions in humans.

This medium was originally described by Slanetz and Bartley for the enumeration of enterococci from water samples using a membrane filtration technique, but it may also be used as a spread plate for the examination of other sample types. Enterococci reduce tetrazolium chloride to the insoluble red dye formazan, producing colonies which are dark red or maroon on the surface of the membrane or agar. This reaction is not exclusive to enterococci, and the count at this stage should be considered presumptive. Colonies may be confirmed as enterococci by demonstrating aesculin hydrolysis using Kanamycin Aesculin Azide Agar (NCM0198).

Specifications
Analyte Enterococci
Brand NEOGEN® Culture Media
Formula
Formula Liter
Tryptose 20.0 g/L
Yeast Extract 5.0 g/L
Glucose 2.0 g/L
Dipotassium Hydrogen Phosphate 4.0 g/L
Sodium Azide 0.4 g/L
2,3,5 Tetrazolium Chloride 0.1 g/L
Agar 12.0 g/L
Grams per Litre 43.50
Litres per 500g 11.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
Plates per 500g 630.00
Preparation
  1. Suspend 43.5 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Cool to 45-50ºC.
  4. DO NOT AUTOCLAVE, PREHEAT OR LEAVE FOR GREATER THAN 2 hours at 45-50ºC.
Previous Item Number Product was previously known as LabM Product: LAB166 Slanetz & Bartley Medium (Membrane Enterococcus Agar)

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