SIM Medium

Item No.  NCM0277A

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SIM Medium is used for the differentiation of microorganisms on the basis of hydrogen sulfide production, indole production, and motility in a laboratory setting. SIM Medium is not intended for use in the diagnosis of disease or other conditions in humans.

Semisolid media have been used extensively in the determination of bacterial motility. The production of hydrogen sulfide, indole formation, and motility are useful diagnostic tests in the identification of Enterobacteriaceae, especially Salmonella spp. and Shigella spp.

In 1940, Sulkin and Willett demonstrated motility, hydrogen sulfide production, and carbohydrate fermentation by members of Salmonella and Shigella groups. They called attention to the “brush-like growth” or motility of the typhoid organisms. Greene et al. used SIM Medium to detect motility in a large series of cultures of typhoid organisms.

Analyte Non-selective
Brand NEOGEN® Culture Media
Formula Liter
Enzymatic Digest of Casein 20.0 g/L
Enzymatic Digest of Animal Tissue 6.1 g/L
Ferric Ammonium Citrate 0.2 g/L
Sodium Thiosulfate 0.2 g/L
Agar 3.5 g/L
Grams per Litre 30.00
Litres per 500g 17.00
Mass 500 g
Package Dimensions 10.20 IN x 10.20 IN x 11.90 IN
Package Weight 15.30 LB
  1. Suspend 30 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC.
Previous Item Number Product was previously known as Acumedia Product: 7221 SIM Medium