Reveal® for E. coli O157:H7
Item No. 700002808 | Catalog No. 9750
- 8-hour enrichment for 25 g samples; 12-hour enrichment for 375 g samples; 20-hour enrichment for both 25 g and 375 g samples
- Irradiated media for easy preparation
| Specifications | ||
|---|---|---|
| Brand | Reveal® | |
| Analyte | E. coli O157:H7 | |
| Platform | Lateral Flow | |
| Result Type | Presence/Absence | |
| Approvals | AOAC Official Method (including 375 g for raw beef product) No. 2000.13 and No. 2000.14 (20-hour enrichment) | |
| Limit of Detection | 1 cfu/analytical unit | |
| Testing Time | 15.00 | |
| Time to Result | 12 20 | |
| Quantity per Package | 20 | |
| Package Dimensions | 13.60 in x 7.80 in x 4.30 in | |
| Package Weight | 1.00 lb | |
Documents
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Technology
This system can utilize various enrichment media to provide E. coli O157:H7 with readily available nutrients and other factors required for its survival and rapid growth. Used with Reveal® media, the system can provide results within 8 or 20 hours for 25 g samples, or results within 12 or 20 hours for 375 g samples.
A portion (120 µL) of the enrichment culture is placed into the round sample port of the Reveal test device. This sample is wicked through a reagent zone that contains specific antibodies (anti-E. coli O157:H7) conjugated to colloidal gold particles. If target antigens are present in the sample, they will bind to the gold-conjugated antibodies. This antigen-antibody complex then leaves the reagent zone and travels through the nitrocellulose membrane that contains a zone of anti-E. coli O157:H7 antibody. The immune complex with gold conjugate is captured and aggregates in this zone, thus displaying a visible line. The remainder of the sample continues to migrate to the end of the membrane where it will eventually become deposited into a waste reservoir.
The reagent zone also contains gold conjugate of a proprietary antigen (color indicator), which is eluted by the sample solution regardless of the presence of E. coli O157:H7 antigen. The gold-conjugated control indicator migrates through the membrane to the negative control capture zone (antibody to the proprietary antigen), where it is captured and aggregated to form a visible line. Regardless of the presence or absence of the E. coli O157:H7 antigen, the control line will form in the control zone, ensuring the test is working properly.
Training
Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.