Reveal® 2.0 for Group D1 Salmonella

1. Tetrathionate Broth Base, Hajna (TT Hajna) (Neogen^® item 7740A) or equivalent
2. Rappaport-Vassiliadis Medium Semisolid Modified (MSRV) (Neogen item 7511A) or equivalent
3. Tryptic Soy Broth (TSB) (Neogen Item 7164A) or equivalent
4. Buffered Peptone Water (BPW) (Neogen item 7418A) or equivalent
5. Reveal 2.0 for SE Media (Neogen Item 9865A)
6. Novobiocin (Neogen item 7985) or equivalent
7. Pipette, 20–200 μL (Neogen item 9276) or equivalent
8. Filtered pipette tips, 100 μL (Neogen item 9389) or equivalent
9. Tubes, 13 x 100 mm glass test tubes (box of 250) (Neogen item 9438A) or equivalent
10. Caps for 13 x 100 mm glass tubes (bag of 250) (Neogen item 9509A) or equivalent
11. Potassium iodide (Sigma no. 221945) or equivalent
12. Iodine (Sigma no. 207772) or equivalent
13. Incubator capable of maintaining 37 ± 1°C (Neogen item 9735) or equivalent
14. Incubator capable of maintaining 42 ± 0.2°C (Neogen item 9735) or equivalent
15. Scale capable of weighing a minimum of 25 g (Neogen item 9427) or equivalent
16. Stomacher-type bags or equivalent
17. Stomacher or equivalent
18. Pipette pump for serological pipettes, 10 mL (Neogen item 9277) or equivalent
19. Pipettes, sterile serological, 10 mL, pack of 100 (Neogen item 9415) or equivalent
20. Timer (3-channel) (Neogen item 9426) or equivalent
21. Test tube rack, 13 mm (Neogen item 9440) or equivalent

Optional Materials (For Additional Confirmation)

1. Reveal Salmonella Enteritidis confirmation bead kit (Neogen item 9723)
2. Phosphate Buffered Saline (PBS) (Neogen item 8425) or equivalent