Reveal® 2.0 for E. coli O157:H7

Item No.  9714

  • 12-20 hour enrichment for 65 g and 375 g samples
  • Irradiated media for easy preparation
This item has shipping restrictions.
$373.42 each
$270.46 each
$200.90 each
In stock
Out of stock
Expect availability in 2-4 weeks
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
The Reveal® 2.0 for E. coli O157:H7 test provides for the rapid recovery of E. coli O157:H7 and E. coli O157:NM organisms in beef matrices facilitating detection and presumptive identification of the target organisms in as little as 12 hours following enrichment.
Brand Reveal®
Analyte E. coli O157:H7
Platform Lateral Flow
Result Type Presence/Absence
Approvals AOAC-RI 011103
Limit of Detection 1 cfu/analytical unit
Testing Time 10.00
Time to Result 8 20
Quantity per Package 20
Package Weight 1.20 lb
  1. 20 Reveal 2.0 for E. coli O157:H7 test devices
  2. 20 transfer pipettes
  3. 20 sample cups
  4. 1 promoter reagent
  1. Stomacher-type bags (Neogen® item 9736)
  2. Scale capable of weighing a minimum of 65 g (Neogen item 9427)
  3. Timer (Neogen item 9416)
  4. Sample cup rack (Neogen item 9475)
  5. Sterile deionized or distilled water
  6. Incubator capable of maintaining 42 ± 1°C (Neogen item 9735)

Test Systems:

NOTE: The Reveal 2.0 for E. coli O157:H7 device requires the use of certain enrichment media and supplies. These can be purchased as part of a test system or individually.

  1. Reveal 2.0 for E. coli O157:H7 complete system (contains devices and media for 20, 65 g samples) (Neogen item 9714)


NOTE: Media can be purchased individually or as part of a test system.

  1. Reveal 2.0 for E. coli O157:H7 media
    1. Unitized (Neogen item 9727)
    2. Bulk 500 g (Neogen item 9728)
    3. 2 kg (Neogen item 9728B)

Optional Materials

  1. Stomacher 400 machine or equivalent


The system utilizes specified enrichment media to provide E. coli O157:H7 with readily available nutrients and other factors required for its survival and rapid growth. Used with Reveal media, the system can provide results in 12 to 20 hours.

A portion (200 µL) of the enriched culture is placed into the sample cup followed by a chemical treatment. The test device is placed into the sample cup during the chemical treatment and is incubated at 42°C for 15 minutes. The sample is wicked through the reagent zone that contains specific anti-E. coli O157:H7 antibodies conjugated to colloidal gold particles.

If antigens are present in the sample, they will bind to the gold-conjugated antibodies. This antigenantibody complex then leaves the reagent zone and travels across the membrane, which contains a zone of anti-E. coli O157:H7 antibodies. The immune complex with gold conjugate is captured and aggregates in this zone, thus displaying a visible line. The remainder of the sample continues to migrate to the end of the membrane where it is deposited into a waste reservoir.

The reagent zone also contains a gold conjugate of a second antigen, which is eluted by the sample regardless of the presence of E. coli O157:H7 antigen to form a line in the control zone, ensuring the test is working properly.


Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.



Our experts are here to help. If you need information beyond what is provided in Validation Reports under the Documents tab, please contact us and we will be happy to assist you. Learn more