Reveal® 2.0 for E. coli O157:H7

SKU No.  700002798  |  Catalog No.  9714

  • 12-20 hour enrichment for 65 g and 375 g samples
  • Irradiated media for easy preparation
This item has shipping restrictions.
$393.00 each
$284.00 each
$211.00 each
In stock
Out of stock
Expect availability in 2-4 weeks
Qty:
Minimum quantity required: 1
This item must be ordered in multiples of one case. Quantity per case: 1
The Reveal® 2.0 for E. coli O157:H7 test provides for the rapid recovery of E. coli O157:H7 and E. coli O157:NM organisms in beef matrices facilitating detection and presumptive identification of the target organisms in as little as 12 hours following enrichment.
Specifications
BrandReveal®
AnalyteE. coli O157:H7
PlatformLateral Flow
Result TypePresence/Absence
ApprovalsAOAC-RI 011103
Limit of Detection1 cfu/analytical unit
Testing Time10.00
Time to Result8 20
Quantity per Package20
Package Dimensions13.60 in x 7.80 in x 4.20 in
Package Weight1.20 lb
  1. 20 Reveal 2.0 for E. coli O157:H7 test devices
  2. 20 transfer pipettes
  3. 20 sample cups
  4. 1 promoter reagent
  1. Stomacher-type bags (Neogen® item 9736)
  2. Scale capable of weighing a minimum of 65 g (Neogen item 9427)
  3. Timer (Neogen item 9416)
  4. Sample cup rack (Neogen item 9475)
  5. Sterile deionized or distilled water
  6. Incubator capable of maintaining 42 ± 1°C (Neogen item 9735)

Test Systems:

NOTE: The Reveal 2.0 for E. coli O157:H7 device requires the use of certain enrichment media and supplies. These can be purchased as part of a test system or individually.

  1. Reveal 2.0 for E. coli O157:H7 complete system (contains devices and media for 20, 65 g samples) (Neogen item 9714)

Media:

NOTE: Media can be purchased individually or as part of a test system.

  1. Reveal 2.0 for E. coli O157:H7 media
    1. Unitized (Neogen item 9727)
    2. Bulk 500 g (Neogen item 9728)
    3. 2 kg (Neogen item 9728B)

Optional Materials

  1. Stomacher 400 machine or equivalent

Documents

To access certificates, please use our technical document search page.

Reveal 2.0 Media E.coli O157-H7 - English - US Reveal® 2.0 for E. coli O157:H7, Complete System - English - US Reveal® 2.0 for E. coli O157:H7 - English - US Reveal® 2.0 for E. coli O157:H7 - Italian - Italy Reveal® 2.0 for E. coli O157:H7 - Portuguese - Brazil Reveal® 2.0 for E. coli O157:H7 - Dutch - Belgium Reveal® 2.0 for E. coli O157:H7 - German - Austria Reveal® 2.0 for E. coli O157:H7 - Chinese Traditional - Taiwan Reveal® 2.0 for E. coli O157:H7 - Japanese - Japan Reveal® 2.0 for E. coli O157:H7 - Bulgarian - Bulgaria Reveal® 2.0 for E. coli O157:H7 - English - Ireland Reveal® 2.0 for E. coli O157:H7 - Spanish - US Reveal® 2.0 for E. coli O157:H7 - Norwegian - Norway Reveal® 2.0 for E. coli O157:H7 - Croatian - Croatia Reveal® 2.0 for E. coli O157:H7 - Greek - Cyprus Reveal® 2.0 for E. coli O157:H7 - Estonian - Estonia Reveal® 2.0 for E. coli O157:H7 - Polish - Poland Reveal® 2.0 for E. coli O157:H7 - English - New Zealand Reveal® 2.0 for E. coli O157:H7 - Thai - Thailand Reveal® 2.0 for E. coli O157:H7 - French - Belgium Reveal® 2.0 for E. coli O157:H7 - Latvian - Latvia Reveal® 2.0 for E. coli O157:H7 - Chinese Simplified - China Reveal® 2.0 for E. coli O157:H7 - Dutch - Netherlands Reveal® 2.0 for E. coli O157:H7 - German - Luxembourg Reveal® 2.0 for E. coli O157:H7 - Hungarian - Hungary Reveal® 2.0 for E. coli O157:H7 - Malay - Malaysia Reveal® 2.0 for E. coli O157:H7 - Slovak - Slovakia Reveal® 2.0 for E. coli O157:H7 - German Reveal® 2.0 for E. coli O157:H7 - English - Australia Reveal® 2.0 for E. coli O157:H7 - English - Malta Reveal® 2.0 for E. coli O157:H7 - Vietnamese - Vietnam Reveal® 2.0 for E. coli O157:H7 - Greek - Greece Reveal® 2.0 for E. coli O157:H7 - Spanish - Mexico Reveal® 2.0 for E. coli O157:H7 - Finnish - Finland Reveal® 2.0 for E. coli O157:H7 - Portuguese - Portugal Reveal® 2.0 for E. coli O157:H7 - Swedish - Sweden Reveal® 2.0 for E. coli O157:H7 - Indonesian - Indonesia Reveal® 2.0 for E. coli O157:H7 - French Reveal® 2.0 for E. coli O157:H7 - Spanish - Spain Reveal® 2.0 for E. coli O157:H7 - English - EU/UK Reveal® 2.0 for E. coli O157:H7 - Czech - Czech Republic Reveal® 2.0 for E. coli O157:H7 - English - Philippines Reveal® 2.0 for E. coli O157:H7 - French - Canada Reveal® 2.0 for E. coli O157:H7 - Spanish - Colombia Reveal® 2.0 for E. coli O157:H7 - Maltese - Malta Reveal® 2.0 for E. coli O157:H7 - English - Canada Reveal® 2.0 for E. coli O157:H7 - Korean - Korea Reveal® 2.0 for E. coli O157:H7 - Slovene - Slovenia Reveal® 2.0 for E. coli O157:H7 - Lithuanian - Lithuania Reveal 2.0 Media E.coli O157-H7 - Bulgarian - Bulgaria Reveal 2.0 Media E.coli O157-H7 - Chinese Simplified - China Reveal 2.0 Media E.coli O157-H7 - Chinese Traditional - Taiwan Reveal 2.0 Media E.coli O157-H7 - Croatian - Croatia Reveal 2.0 Media E.coli O157-H7 - Czech - Czech Republic Reveal 2.0 Media E.coli O157-H7 - Danish - Denmark Reveal 2.0 Media E.coli O157-H7 - Dutch - Belgium Reveal 2.0 Media E.coli O157-H7 - Dutch - Netherlands Reveal 2.0 Media E.coli O157-H7 - English - Australia Reveal 2.0 Media E.coli O157-H7 - English - Canada Reveal 2.0 Media E.coli O157-H7 - English - EU/UK Reveal 2.0 Media E.coli O157-H7 - English - Ireland Reveal 2.0 Media E.coli O157-H7 - English - Malta Reveal 2.0 Media E.coli O157-H7 - English - New Zealand Reveal 2.0 Media E.coli O157-H7 - English - Philippines Reveal 2.0 Media E.coli O157-H7 - Estonian - Estonia Reveal 2.0 Media E.coli O157-H7 - Finnish - Finland Reveal 2.0 Media E.coli O157-H7 - French Reveal 2.0 Media E.coli O157-H7 - French - Belgium Reveal 2.0 Media E.coli O157-H7 - French - Canada Reveal 2.0 Media E.coli O157-H7 - German Reveal 2.0 Media E.coli O157-H7 - German - Austria Reveal 2.0 Media E.coli O157-H7 - German - Luxembourg Reveal 2.0 Media E.coli O157-H7 - Greek - Cyprus Reveal 2.0 Media E.coli O157-H7 - Greek - Greece Reveal 2.0 Media E.coli O157-H7 - Hungarian - Hungary Reveal 2.0 Media E.coli O157-H7 - Indonesian - Indonesia Reveal 2.0 Media E.coli O157-H7 - Italian - Italy Reveal 2.0 Media E.coli O157-H7 - Japanese - Japan Reveal 2.0 Media E.coli O157-H7 - Korean - Korea Reveal 2.0 Media E.coli O157-H7 - Latvian - Latvia Reveal 2.0 Media E.coli O157-H7 - Lithuanian - Lithuania Reveal 2.0 Media E.coli O157-H7 - Malay - Malaysia Reveal 2.0 Media E.coli O157-H7 - Maltese - Malta Reveal 2.0 Media E.coli O157-H7 - Norwegian - Norway Reveal 2.0 Media E.coli O157-H7 - Polish - Poland Reveal 2.0 Media E.coli O157-H7 - Portuguese - Brazil Reveal 2.0 Media E.coli O157-H7 - Portuguese - Portugal Reveal 2.0 Media E.coli O157-H7 - Romanian - Romania Reveal 2.0 Media E.coli O157-H7 - Slovak - Slovakia Reveal 2.0 Media E.coli O157-H7 - Slovene - Slovenia Reveal 2.0 Media E.coli O157-H7 - Spanish - Colombia Reveal 2.0 Media E.coli O157-H7 - Spanish - Mexico Reveal 2.0 Media E.coli O157-H7 - Spanish - Spain Reveal 2.0 Media E.coli O157-H7 - Spanish - US Reveal 2.0 Media E.coli O157-H7 - Swedish - Sweden Reveal 2.0 Media E.coli O157-H7 - Thai - Thailand Reveal 2.0 Media E.coli O157-H7 - Vietnamese - Vietnam Reveal® 2.0 for E. coli O157:H7 - Danish - Denmark Reveal® 2.0 for E. coli O157:H7, Complete System - Bulgarian - Bulgaria Reveal® 2.0 for E. coli O157:H7, Complete System - Chinese Simplified - China Reveal® 2.0 for E. coli O157:H7, Complete System - Chinese Traditional - Taiwan Reveal® 2.0 for E. coli O157:H7, Complete System - Croatian - Croatia Reveal® 2.0 for E. coli O157:H7, Complete System - Czech - Czech Republic Reveal® 2.0 for E. coli O157:H7, Complete System - Danish - Denmark Reveal® 2.0 for E. coli O157:H7, Complete System - Dutch - Belgium Reveal® 2.0 for E. coli O157:H7, Complete System - Dutch - Netherlands Reveal® 2.0 for E. coli O157:H7, Complete System - English - Australia Reveal® 2.0 for E. coli O157:H7, Complete System - English - Canada Reveal® 2.0 for E. coli O157:H7, Complete System - English - EU/UK Reveal® 2.0 for E. coli O157:H7, Complete System - English - Ireland Reveal® 2.0 for E. coli O157:H7, Complete System - English - Malta Reveal® 2.0 for E. coli O157:H7, Complete System - English - New Zealand Reveal® 2.0 for E. coli O157:H7, Complete System - English - Philippines Reveal® 2.0 for E. coli O157:H7, Complete System - Estonian - Estonia Reveal® 2.0 for E. coli O157:H7, Complete System - Finnish - Finland Reveal® 2.0 for E. coli O157:H7, Complete System - French Reveal® 2.0 for E. coli O157:H7, Complete System - French - Belgium Reveal® 2.0 for E. coli O157:H7, Complete System - French - Canada Reveal® 2.0 for E. coli O157:H7, Complete System - German Reveal® 2.0 for E. coli O157:H7, Complete System - German - Austria Reveal® 2.0 for E. coli O157:H7, Complete System - German - Luxembourg Reveal® 2.0 for E. coli O157:H7, Complete System - Greek - Cyprus Reveal® 2.0 for E. coli O157:H7, Complete System - Greek - Greece Reveal® 2.0 for E. coli O157:H7, Complete System - Hungarian - Hungary Reveal® 2.0 for E. coli O157:H7, Complete System - Indonesian - Indonesia Reveal® 2.0 for E. coli O157:H7, Complete System - Italian - Italy Reveal® 2.0 for E. coli O157:H7, Complete System - Japanese - Japan Reveal® 2.0 for E. coli O157:H7, Complete System - Korean - Korea Reveal® 2.0 for E. coli O157:H7, Complete System - Latvian - Latvia Reveal® 2.0 for E. coli O157:H7, Complete System - Lithuanian - Lithuania Reveal® 2.0 for E. coli O157:H7, Complete System - Malay - Malaysia Reveal® 2.0 for E. coli O157:H7, Complete System - Maltese - Malta Reveal® 2.0 for E. coli O157:H7, Complete System - Norwegian - Norway Reveal® 2.0 for E. coli O157:H7, Complete System - Polish - Poland Reveal® 2.0 for E. coli O157:H7, Complete System - Portuguese - Brazil Reveal® 2.0 for E. coli O157:H7, Complete System - Portuguese - Portugal Reveal® 2.0 for E. coli O157:H7, Complete System - Slovak - Slovakia Reveal® 2.0 for E. coli O157:H7, Complete System - Slovene - Slovenia Reveal® 2.0 for E. coli O157:H7, Complete System - Spanish - Colombia Reveal® 2.0 for E. coli O157:H7, Complete System - Spanish - Mexico Reveal® 2.0 for E. coli O157:H7, Complete System - Spanish - Spain Reveal® 2.0 for E. coli O157:H7, Complete System - Spanish - US Reveal® 2.0 for E. coli O157:H7, Complete System - Swedish - Sweden Reveal® 2.0 for E. coli O157:H7, Complete System - Thai - Thailand Reveal® 2.0 for E. coli O157:H7, Complete System - Vietnamese - Vietnam

Technology

The system utilizes specified enrichment media to provide E. coli O157:H7 with readily available nutrients and other factors required for its survival and rapid growth. Used with Reveal media, the system can provide results in 12 to 20 hours.

A portion (200 µL) of the enriched culture is placed into the sample cup followed by a chemical treatment. The test device is placed into the sample cup during the chemical treatment and is incubated at 42°C for 15 minutes. The sample is wicked through the reagent zone that contains specific anti-E. coli O157:H7 antibodies conjugated to colloidal gold particles.

If antigens are present in the sample, they will bind to the gold-conjugated antibodies. This antigenantibody complex then leaves the reagent zone and travels across the membrane, which contains a zone of anti-E. coli O157:H7 antibodies. The immune complex with gold conjugate is captured and aggregates in this zone, thus displaying a visible line. The remainder of the sample continues to migrate to the end of the membrane where it is deposited into a waste reservoir.

The reagent zone also contains a gold conjugate of a second antigen, which is eluted by the sample regardless of the presence of E. coli O157:H7 antigen to form a line in the control zone, ensuring the test is working properly.

Training

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training to provide you with the documentation needed for audit traceability.

Support

Validation

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