Reinforced Clostridial Medium

Item No.  NCM0102A

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Reinforced Clostridial Medium is used for the cultivation of anaerobes from food. This medium conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. Reinforced Clostridial Medium (RCM) is recommended by the Harmonized Pharmacopoeia for the selective enrichment of Clostridia from non-sterile samples and conforms to USP/EP/JP performance specifications. Peptone, beef and yeast extract provide a source of nitrogen, essential vitamins and amino acids. Starch aids the detoxification of harmful metabolites and glucose is a fermentable carbohydrate. Sodium chloride provides osmotic balance and sodium acetate acts as a buffer. L-Cysteine hydrochloride acts as a reducing agent to create an anaerobic environment and maintain a low Eh. This is aided by the low level of agar which reduces the oxygen permeability through the medium. According to the Harmonized Pharmacopoeia, Reinforced Medium for Clostridia is used as a selective enrichment broth, with subculture performed onto Columbia Agar.
Analyte Clostridium spp.
Brand NEOGEN® Culture Media
Formula Liter
Beef Extract 10.0 g/L
Peptone 10.0 g/L
Yeast Extract 3.0 g/L
Soluble Starch 1.0 g/L
Glucose Monohydrate 5.0 g/L
Cysteine Hydrochloride 0.5 g/L
Sodium Chloride 5.0 g/L
Sodium Acetate 3.0 g/L
Agar 0.5 g/L
Grams per Litre 38.00
Litres per 500g 13.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Suspend 38 grams of powder in 1 liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium, if necessary.
  3. Autoclave at 121°C for 15 minutes.
Previous Item Number Product was previously known as Acumedia Product: 7714 Reinforced Clostridial Medium; and LabM Product: HP011 Reinforced Medium for Clostridia (USP/EP/JP)