Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified
SKU No.  700003668  | Catalog No.  NCM0289A
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Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified

SKU No.  700003668  |  Catalog No.  NCM0289A

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Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified is a modification of Rappaport-Vassiliadis enrichment broth for detecting motile Salmonella spp.in food products. The original research on MSRV Medium revealed a semisolid could be used as a rapid and sensitive test for isolating motile Salmonella from food products following pre-enrichment or selective enrichment. The semisolid medium allows motility to be detected as halos of growth around the original point of inoculation.

MSRV Medium is recommended by the European Chocolate Manufacturer’s Association. A collaborative study performed with support of American Cocoa Research Institute and the Canadian Chocolate Manufacturer’s Association resulted in first action adoption of the MSRV method by AOAC International.

Specifications
BrandNeogen® Culture Media
PlatformDehydrated Culture Media
Formula
FormulaLiter
Enzymatic Digest of Casein4.59 g/L
Casein Acid Hydrolysate4.59 g/L
Sodium Chloride7.34 g/L
Potassium Dihydrogen Phosphate1.47 g/L
Magnesium Chloride, Anhydrous10.93 g/L
Malachite Green Oxalate0.037 g/L
Agar2.70 g/L
Grams per Liter31.60
Liters per 500 g15.80
Plates per 500 g1,053.00
Preparation
  1. Suspend 31.6 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool medium to 45 - 50°C and aseptically add 10 ml of Novobiocin Supplement (7985).
  5. Mix well and dispense into petri dishes.
Previous Item NumberProduct was previously known as Acumedia product: 7511 Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified

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