Pseudomonas Agar Base

Item No.  NCM0083A

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Pseudomonas Agar Base is used for the selective and differential isolation of Pseudomonas sp. using CN or CFC supplementation and is not intended for use in the diagnosis of disease or other conditions in humans.

Pseudomonas Agar Base was developed to be supplemented with CN X107 (Cetrimide & Nalidixic Acid) as per ISO 16266-2:2018, or with CFC X108 (Cetrimide, Fusidate, & Cephaloridine) as per ISO 13720:2010. This base formula is a modification of King’s A Medium.

The CN Supplement is recommended for the isolation of Pseudomonas aeruginosa, where the addition of Nalidixic Acid and the reduction of Cetrimide improved recovery. Pseudomonas Agar Base, with the addition of CN Supplement, demonstrated enhanced pigment formation of Ps. aeruginosa and increased inhibition of Klebsiella, Proteus, and Providencia spp. Pseudomonas Agar Base with the addition of CFC Supplement is recommended for the selective isolation of Pseudomonas spp. Mead and Adams reduced Cetrimide, permitting growth of all pigmented and non-pigmented psychrophilic pseudomonads. The antimicrobics, Fusidate and Cephaloridine, were added to increase the selectivity of the medium. This formulation is used for Pseudomonas spp. from chilled foods and processing plants.

Pseudomonas Agar Base is recommended by ISO for the enumeration of Pseudomonas spp. from meat and meat products according to ISO 13720, and for the detection of enumeration of Pseudomonas aeruginosa spp. in water according to ISO 16266.

Specifications
Brand Neogen® Culture Media
Analyte Pseudomonas spp.
Platform Dehydrated Culture Media
Mass 500 g
Formula
Formula Liter
Enzymatic Digest of Gelatin 16.0 g/L
Enzymatic Digest of Casein 10.0 g/L
Potassium Sulfate 10.0 g/L
Magnesium Chloride 1.4 g/L
Agar 11.0 g/L
Grams per Liter 48.40
Liters per 500 g 10.00
Plates per 500 g 560.00
Preparation
  1. Suspend 48.4 grams of the medium and 10 mL of glycerol in one liter of purified water and bring to a boil.
  2. Autoclave at 121ºC for 15 minutes.
  3. Cool to 45-50ºC.
  4. Aseptically add 2 vials of CN Supplement (X107) and mix well before dispensing.
Previous Item Number Product was previously known as Acumedia Product: 9222 Pseudomonas Agar Base ; and LabM Product: LAB108 Pseudomonas Agar Base
Package Weight 1.35 lb

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