PPLO Broth Base without Crystal Violet

Item No.  NCM0113A

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PPLO Broth Base without Crystal Violet is used with enrichments for the isolation and cultivation of Mycoplasma spp. in a laboratory setting. PPLO Broth without Crystal Violet is not intended for the use in the diagnosis of disease or other conditions in humans.

PPLO Broth Base without Crystal Violet is prepared according to the formula described by Morton and Lecce. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as “pleuropneumonia-like organism” or PPLO. Mycoplasmas belong to the class of Mollicutes “soft skin”, which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 μm.

Analyte Mycoplasma spp.
Brand NEOGEN® Culture Media
Formula Liter
Heart Infusion 6.0 g/L
Yeast Enriched Peptone 10.0 g/L
Sodium Chloride 5.0 g/L
Grams per Litre 21.00
Litres per 500g 24.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 21 grams of the medium in 100 mL of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes.
  4. Cool to 45-50ºC and aseptically add 300 mL of the appropriate sterile serum supplement.
  5. Mix thoroughly.
Previous Item Number Product was previously known as Acumedia Product: 0113 PPLO Broth Base without Crystal Violet; and LabM Product: LAB229 PPLO Broth Base without Crystal Violet



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