Phosphate Buffer, pH 7.2

Item No.  NCM0223A

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Minimum quantity required: 1
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Phosphate Buffer, pH 7.2 is used for the preparation of microbiological dilution blanks in a laboratory setting. Phosphate Buffer, pH 7.2 is not intended for use in the diagnosis of disease or other conditions in humans.

The formula for phosphate buffer is specified by the American Public Health Association (APHA) for use in diluting test samples. Phosphate Buffer, pH 7.2 is specified for use in diluting water, dairy products and food for microbiological methods. In the examination of water and dairy products the addition of magnesium chloride is recommended.

This buffer is also referred to as Butterfield’s Buffered Phosphate Diluent and recommended for examination of food. Phosphate Buffer, pH 7.2 stabilizes the pH of water used for dilutions.

Analyte Non-selective
Brand NEOGEN® Culture Media
Formula Liter
Potassium Dihydrogen Phosphate 26.22 g/L
Sodium Carbonate 7.78 g/L
Grams per Litre 34.00
Litres per 500g 15.00
Mass 500 g
Package Dimensions 4.60 IN x 2.90 IN x 8.60 IN
Package Weight 1.35 LB
  1. Dissolve 34 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121ºC for 15 minutes. Store under refrigeration.
Previous Item Number Product was previously known as Acumedia Product: 7380 Phosphate Buffer, pH 7.2